Discuss : Effect of antiandrogens on the tropism of seasonal human coronaviruses • Discussing the background of the project: o COVID -> Variants -> Alpha, beta, delta, omicron (BA1, BA2, BA4, BA5),wuhano IFITMs 1,2,3. Role, importance, what is know about SARS- COV-2 and IFITM, overexpress, under express etco Diagram of omicron entry showing IFITMS (example below)o Explain Pseudotypes (using HEK293 T cells)o Titration using A549+ACE2 (testing with Camostat, E64D, amfoterridn IFITMso Transfect with IFITM1,2,3 (A549+ACE2)o Neg control (no- spike control)o Positive control VSV-Go Western blot analysiso Luciferase signal
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