Explain the relation between genomic DNA and RNA, describe what an intron is. What is the purpose of the experiment? Shortly summarize the outcome of the sequence analysis of rad21, do you think it has an intron? Explain the experimental design and how it should allow you to detect the absence or presence of the predicted intron. What is the function of each of the main steps in the protocol? What is the expected result in case of the intron being present? What if the intron is absent? Describe any deviations (if any) from the protocol. How much RNA did you purify? How much genomic DNA? Present your calculations. Present the picture of the gel and label it properly: Each lane should be labeled, mark the sizes of the DNA ladder. Provide a figure legend. Determine the sizes of the bands that you can see and mention these in the text. Most importantly: describe and Interpret your results in the main text, talk the reader through what you conclude from your data and why. Explain your reasoning. If you failed to get results during the practical or if you results differ from what you expected, try to give an explanation as to what you think went wrong.
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